Journal of University of Anbar for Pure Science

Genetic identification of Opr I and Opr L Genes in Pseudomonas aeruginosa isolated from different local sources.

Mohammed E. Altaai; Jamal A. Alhadithy; Wissam K. Al Swediawy

Volume 11, Issue 1 , April 2017, , Page 20-29

https://doi.org/10.37652/juaps.2017.141449

Abstract

P.aeruginosa bacteria are opportunistic pathogens capable of infection almost every tissue in the body as a result of possessing a large variety of virulence factors which significantly ... Read More ... P.aeruginosa bacteria are opportunistic pathogens capable of infection almost every tissue in the body as a result of possessing a large variety of virulence factors which significantly contribute to the pathogenicity of the host events. In the current study was to collect 119 samples from different clinical and environmental sources. to investigate the spread of bacteria P.aeruginosa and to study the virulence factors by a sample of 90 of those patients in several Hospitals in the city of Baghdad, including 33 samples of burns infections, 22 samples of wound infections, 17 samples of Otitis media infections, and 11 samples for urinary tract infections as well as 7 isolates belonging to patients with cystic fibrosis, The environmental samples included 10 samples of soil, 10 samples of water, and 9 samples of theaters. Diagnosed develop isolated after planted on different growth media through phenotypic traits and microscopic examinations and confirmed diagnosis using API 20E kit, as well as molecular diagnostics definitive diagnosis of isolates that give a positive result as bacteria P.aeruginosa during the previous tests were depending on the gene diagnosis 16SrDNA with a special bacterial sequence of P.aeruginosa. Diagnostic results showed that 61 isolated a 51% belonging for the type of P.aeruginosa of the total clinical and environmental samples that were divided on 15 isolates 24.6% of cases of burns infections, 13 isolates 21.4% of cases of wound infections, 10 isolates 16.3% of cases of Otitis media infections, 5 isolates 8.3% of cystic fibrosis, 4 isolates 6.5% of cases of urinary tract infections, 6 isolates 9.8% of soil, 4 isolates 6.5% of water, and 4 isolates 6.5% of the galleries operations. The results of the phenotypic detections of some virulence factors showed that 95% of the isolates were producing hemolysin enzyme type β –hemolysis, while it was 93.44% of the isolates producing protease enzyme and to varying degrees, and the percentage of isolates producing pyocyanin pigment was 73.13%. The prevalence of some virulence factors of P.aeruginosa was investigated in both clinical and environmental isolates by molecular genetic methods, included these genes each of the genes encoded of fatty proteins Opr I, Opr L. The results showed the existence of genes ( Opr I ) increased by 100%, while the gene Opr L ratio was 96.72% within the genetic structure of the isolates studied.

Genetic Diagnosis of tox A and las B Genes in Pseudomonas aeruginosa isolated from different clinical sources.

Mohammed E. Altaai; Jamal A. Alhadithy; Wissam K. Al Swediawy

Volume 10, Issue 3 , December 2016, , Page 47-56

https://doi.org/10.37652/juaps.2016.135141

Abstract

Histological variation resulting from the injection of bacteria P.aeruginosa and crud protease enzyme and partially purified in white mice

Wafaa T. Radef; Ammen S. Badwi; Dhafer F. Al-Rawi

Volume 10, Issue 2 , August 2016, , Page 72-80

https://doi.org/10.37652/juaps.2016.132601

Abstract

Studied the effects Histopathological of stuck bacterial P.aeruginosa and enzyme protease crude and Partially purified in the liver and kidney of rats injected and by 0.1 and 0.05 μl ... Read More ...

Molecular characterization of Pseudomonas aeruginosa Isolates Isolated from Clinical Patients by Using RAPD-PCR Technique.

Akeel H. AL-Assie; Adnan F. Al-azawy; Wakas S. Al-Dori

Volume 6, Issue 3 , December 2012, , Page 27-35

https://doi.org/10.37652/juaps.2012.78439

Abstract

The aim of the present study was the molecular characterization and the evaluation of variability and genetic relationship of six Pseudomonas aeruginosa isolates using PCR-based Randomly ... Read More ... The aim of the present study was the molecular characterization and the evaluation of variability and genetic relationship of six Pseudomonas aeruginosa isolates using PCR-based Randomly Amplified Polymorphic DNA (RAPD) technique. A total number of 86 samples were collected from patients that hospitalized in Tikrit Teaching Hospital in Tikrit city. These samples were taken from patients basing on the sources of infections, the isolates were taken from: wounds, ear, burns, urine, sputum, and eyes infections. Using enrichment, selective media, and biochemical tests, that characterized and identified as P. aeruginosa. Genomic DNA was extracted from six P. aeruginosa isolates isolated from these different sources. These genomic DNA samples were found to have a suitable concentration and purity for RAPD-PCR analysis. RAPD-PCR technique was performed using 15 different Operon random primers. Eleven primers gave successful amplification results in repeated experiments. As a result, the total number of amplified bands was 270 and the total number of polymorphic bands was 234. The highest number of polymorphic bands (39 bands) was produced by primer OPX-01. The primer efficiency ranged from 3.70 (primer OPA-11) to 14.44 (primer OPX-01) and the discriminatory value ranged from 1.70% (primer OPA-11) to 16.66% (primer OPX-01). In addition, genetic distance and cluster analysis among different P. aeruginosa isolates were estimated by using UPGMA computer program basing on RAPD-PCR banding patterns that obtained in this study. These results suggesting that possible and frequent occurrence of mutations in DNA sequencing P. aeruginosa bacteria from different sources and locations. This study has proved existence genetic differences (DNA polymorphism) among the six P. aeruginosa isolates isolated from different sources. Therefore, we can say that RAPD technique could be an efficient technique for studying the molecular characterization and the epidemiology of P. aeruginosa bacteria.

Journal of University of Anbar for Pure Science

Articles in Press

Current Issue

Volume 17 (2023)

Volume 16 (2022)

Volume 15 (2021)

Volume 14 (2020)

Volume 13 (2019)

Volume 12 (2018)

Volume 11 (2017)

Volume 10 (2016)

Volume 9 (2015)

Volume 8 (2014)

Volume 7 (2013)

Volume 6 (2012)

Volume 5 (2011)

Volume 4 (2010)

Volume 3 (2009)

Volume 2 (2008)

Volume 1 (2007)

Keywords = aeruginosa

Genetic identification of Opr I and Opr L Genes in Pseudomonas aeruginosa isolated from different local sources.

Abstract

Genetic Diagnosis of tox A and las B Genes in Pseudomonas aeruginosa isolated from different clinical sources.

Abstract

Histological variation resulting from the injection of bacteria P.aeruginosa and crud protease enzyme and partially purified in white mice

Abstract

Molecular characterization of Pseudomonas aeruginosa Isolates Isolated from Clinical Patients by Using RAPD-PCR Technique.

Abstract